| Autor: |
Eliza C, Small, Danielle N, Maryanski, Keli L, Rodriguez, Kevin J, Harvey, Michael-C, Keogh, Andrea L, Johnstone |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2261 |
| ISSN: |
1940-6029 |
| Popis: |
Chromatin immunoprecipitation (ChIP) is a method used to examine the genomic localization of a target of interest (e.g., proteins, protein posttranslational modifications, or DNA elements). As ChIP provides a snapshot of in vivo DNA-protein interactions, it lends insight to the mechanisms of gene expression and genome regulation. This chapter provides a detailed protocol focused on native-ChIP (N-ChIP), a robust approach to profile stable DNA-protein interactions. We also describe best practices for ChIP , including defined controls to ensure specific and efficient target enrichment and methods for data normalization. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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