Effect of MTA and CEM on Mineralization-Associated Gene Expression in Stem Cells Derived from Apical Papilla

Autor:	Hajizadeh, Niusha, Madani, Zahra Sadat, Zabihi, Ebrahim, Golpour, Moniyreh, Zahedpasha, Amir, Mohammadnia, Mousa
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	Biomaterials Retrograde Root Filling Materials Relative Gene Expression Mineral Trioxide Aggregate Original Article Stem Cells from Apical Papilla Calcium-Enriched Mixture (CEM Cement)
Zdroj:	Iranian Endodontic Journal
ISSN:	2008-2746 1735-7497
Popis:	Introduction: This study assessed the effect of mineral trioxide aggregate (MTA) and calcium-enriched mixture (CEM) cement on odontogenic differentiation and mineralization of stem cells. Methods and Materials: After confirmation of stemness and homogeneity of stem cells derived from apical papilla (SCAPs) using flow cytometry, the cells were exposed for 3 weeks to either osteogenic medium (OS) or CEM extract+OS (CEM+OS) or MTA extract in OS (MTA+OS) or DMEM based regular culture media (negative control). Relative expression of alkaline phosphatase (ALP), dentine sialophosphoprotein (DSPP), osteocalcin (OSC), and osterix (SP7) were measured at days 14 and 21 using RT-qPCR method. At the same time points Alizarin Red staining method was used to assess mineralization potential of SCAPS. Gene expression changes analysis were made automatically using REST® software and a P
Databáze:	OpenAIRE
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