Spatiotemporal properties of cytoplasmic cyclic AMP gradients can alter the turning behaviour of neuronal growth cones

Autor:	Sebastian, Munck, Peter, Bedner, Thierry, Bottaro, Hartmann, Harz
Rok vydání:	2004
Předmět:	Neurons Cytoplasm Time Factors Cell Movement Growth Cones Cyclic AMP Animals Humans Cattle Chick Embryo Cells Cultured HeLa Cells
Zdroj:	The European journal of neuroscience. 19(4)
ISSN:	0953-816X
Popis:	Growth cones, the terminal structures of elongating neurites, use extracellular guidance information in order to navigate to appropriate target cells. The directional information of guidance cues is transduced to a cytoplasmic gradient of messenger molecules across the growth cone leading to rearrangements of the cytoskeleton. One messenger molecule regulating growth cone turning is cAMP, which is also known to be sufficient to direct growth cone attraction. Cytoplasmic cAMP gradients have been generated in the present study by photolysing caged cAMP with UV light focused on one side of growth cones of chick sensory neurons. Using this method we show that only specific time patterns of pulsed cAMP release are capable of inducing growth cone turning whereas others, which release the same amount of cAMP, are ineffective. Theoretical calculations show that diverse time patterns produce different intracellular gradients, which were visualized directly in HeLa cells expressing cAMP-sensitive ion channels as a reporter system. Together these data indicate that the spatiotemporal properties of the intracellular gradient are crucial for growth cone turning.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::011bc30731e92fb33b49774b68d1c8d3 https://pubmed.ncbi.nlm.nih.gov/15009126 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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