| Autor: |
A Iu, Misharin, A S, Krylov, C, Alquier, H, LaFont, A Ia, Shteĭnshneĭder, V A, Kosykh, A D, Morozkin |
| Rok vydání: |
1997 |
| Předmět: |
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| Zdroj: |
Bioorganicheskaia khimiia. 23(4) |
| ISSN: |
0132-3423 |
| Popis: |
beta-(2-Hydroxyethoxy)-5 alpha-cholest-8(14)-en-15-one, a synthetic inhibitor of cholesterol biosynthesis, was shown to exhibit a high affinity to oxysterol binding protein. This was proved by ultracentrifugation of the protein fraction from rabbit liver in the presence of the 3H-labeled inhibitor, 3 beta-(2-hydroxy-2-[3H]ethoxy)-5 alpha-cholest-8(14)-en-15-one, or by the substitution of the [3H]-25-hydroxycholesterol in its complex with the oxysterol binding protein. In human hepatoma Hep G2 cells, the inhibitor decreased activity of 3-hydroxy-3-methylglutaryl CoA reductase [ID50 (2.7 +/- 0.7) x 10(-5) M] and was transformed into 3 beta-[2-(9-Z-octadecenoyloxy)ethoxy]-5 alpha-cholest-8(14)-en-15-one. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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