| Autor: |
Sita S, Chandrasekaran, Shreeya, Agrawal, Alison, Fanton, Aditya R, Jangid, Bérénice, Charrez, Arturo M, Escajeda, Sungmin, Son, Roger, Mcintosh, Huyen, Tran, Abdul, Bhuiya, María Díaz, de León Derby, Neil A, Switz, Maxim, Armstrong, Andrew R, Harris, Noam, Prywes, Maria, Lukarska, Scott B, Biering, Dylan C J, Smock, Amanda, Mok, Gavin J, Knott, Qi, Dang, Erik, Van Dis, Eli, Dugan, Shin, Kim, Tina Y, Liu, Erica A, Moehle, Katherine, Kogut, Brenda, Eskenazi, Eva, Harris, Sarah A, Stanley, Liana F, Lareau, Ming X, Tan, Daniel A, Fletcher, Jennifer A, Doudna, David F, Savage, Alisha, Baldwin |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Nature biomedical engineering. 6(8) |
| ISSN: |
2157-846X |
| Popis: |
Rapid nucleic acid testing is central to infectious disease surveillance. Here, we report an assay for rapid COVID-19 testing and its implementation in a prototype microfluidic device. The assay, which we named DISCoVER (for diagnostics with coronavirus enzymatic reporting), involves extraction-free sample lysis via shelf-stable and low-cost reagents, multiplexed isothermal RNA amplification followed by T7 transcription, and Cas13-mediated cleavage of a quenched fluorophore. The device consists of a single-use gravity-driven microfluidic cartridge inserted into a compact instrument for automated running of the assay and readout of fluorescence within 60 min. DISCoVER can detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in saliva with a sensitivity of 40 copies μl |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full text from SpringerLink