SP6 RNA polymerase containing vaccinia virus for rapid expression of cloned genes in tissue culture

Autor:	T B, Usdin, M J, Brownstein, B, Moss, S N, Isaacs
Rok vydání:	1993
Předmět:	DNA Viral Gene Expression Bacteriophages Vaccinia virus DNA-Directed RNA Polymerases Cloning Molecular Promoter Regions Genetic Transfection beta-Galactosidase Recombinant Proteins Plasmids
Zdroj:	BioTechniques. 14(2)
ISSN:	0736-6205
Popis:	A hybrid transient expression system, in which tissue culture cells are infected with a recombinant vaccinia virus encoding bacteriophage DNA-dependent RNA polymerase and transfected with a plasmid containing a cloned gene behind the bacteriophage promoter, allows rapid high-level expression in nearly 100% of the cells. In order to extend this system to clones from libraries containing SP6 promoters, a new vaccinia virus was constructed encoding bacteriophage SP6 RNA polymerase.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::00b6109f38dd4e8550b8d2fea665dcf1 https://pubmed.ncbi.nlm.nih.gov/8431286 Zobrazit plný text záznamu