| Autor: |
A V, Zlatska, A E, Rodnichenko, O S, Gubar, D O, Zubov, S N, Novikova, R G, Vasyliev |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Experimental oncology. 39(3) |
| ISSN: |
1812-9269 |
| Popis: |
We aimed to study biological properties of human endometrial stromal cells in vitro.The endometrium samples (n = 5) were obtained by biopsy at the first phase of the menstrual cycle from women with endometrial hypoplasia. In all cases, a voluntary written informed consent was obtained from the patients. Endometrial fragments were dissociated by enzymatic treatment. The cells were cultured in DMEM/F12 supplemented with 10% FBS, 2 mМ L-glutamine and 1 ng/ml FGF-2 in a multi-gas incubator at 5% COPrimary population of endometrial cells was heterogeneous and contained cells with fibroblast-like and epithelial-like morphology, but at P3 the majority of cell population had fibroblast-like morphology. The cells possessed typical for MSCs phenotype CD90Thus, cultured endometrial stromal cells meet minimal ISCT criteria for MSC. Proliferative potential, karyotype stability, multilineage plasticity and secretome profile make eMSC an attractive object for the regenerative medicine use. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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