Anti-invasive effect of contortrostatin, a snake venom disintegrin, and TNF-alpha on malignant glioma cells
| Autor: | S, Schmitmeier, F S, Markland, T C, Chen |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Tumor Necrosis Factor-alpha
Disintegrins Gold Colloid Fibronectins Drug Combinations Carcinogens Cell Adhesion Tumor Cells Cultured Humans Tetradecanoylphorbol Acetate Drug Therapy Combination Neoplasm Invasiveness Proteoglycans Collagen Laminin Vitronectin Drug Screening Assays Antitumor Glioblastoma Protein Kinase C |
| Zdroj: | Anticancer research. 20(6B) |
| ISSN: | 0250-7005 |
| Popis: | The snake venom disintegrin contortrostatin has been shown to bind to integrins alpha IIb beta 3, alpha v beta 3, alpha v beta 5, and alpha 5 beta 1 and to exert an anti-tumor activity in vitro and in vivo. The cytokine TNF-alpha has been demonstrated to have anti-invasive properties in vitro.The human glioblastoma cell line T98G was treated with controtrostatin or colloidal gold-TNF-alpha (CG-TNF-alpha) alone, or in combination. Vitronectin- and fibronectin-dependent adhesion of untreated and treated glioma cells was studied and compared. Invasion through a reconstituted basement membrane (Matrigel) was also examined.Although both contortrostatin and CG-TNF-alpha inhibited invasion of T98G cells through Matrigel, the mechanism of inhibition appears to be different. Contortrostatin significantly decreased cell adhesion to vitronectin and fibronectin; CG-TNF-alpha did not. Contortrostatin binds to T98G integrins in an RGD-dependent manner, whereas protein kinase C (PKC) appears to be involved in CG-TNF-alpha actions, leading to inhibition of cell invasion. The efficiency of contortrostatin in inhibiting cell invasion was enhanced by combination with CG-TNF-alpha.The combined use of contortrostatin and CG-TNF-alpha may have potential for malignant glioma therapy by effectively inhibiting glioma cell invasion. |
| Databáze: | OpenAIRE |
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