| Autor: |
Feng-xia, Bai, Shi-feng, Lou |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 27(1) |
| ISSN: |
1007-8738 |
| Popis: |
to construct a eukaryolic expression plasmid of Ppic9k-IL3-Linker-PE38KDE.the IL3 and PE38KDEL gene were amplified by polymerase chain reaction (PCR) and cloned into the eukaryolic expression plasmid Ppic9k-Linker constructed after being sequenced.The recombinant vector confirmed by restriction endonucleases digestion, coenobium PCR and DNA sequence analysis showed that the prokaryotic expression vector Ppic9k-IL3-Linker-PE38KDEL was constructed successfully.the fusion gene IL3-PE38KDEL is successfully constructed, which laid a solid foundation for the further research. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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