| Autor: |
V Ia, Brodskiĭ, A V, Vasil'ev, V V, Terskikh, N D, Zvezdina, V I, Fateeva, L A, Mal'chenko, E V, Kiseleva, E I, Bueverova |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Ontogenez. 43(3) |
| ISSN: |
0475-1450 |
| Popis: |
A comparative study was performed of dense 5-hour cultures of rat hepatocytes and equal-density cultures of mesenchymal stromal cells (MSC) isolated from human adipose tissue of rat bone marrow. The cells were grown on collagen-coated class slides in serum-free medium. Unlike in hepatocytes, no rhythm of protein synthesis was initially revealed in MSC, but such a rhythm manifested itself when the culture medium was supplemented with melatonin (2 nM, 5 min). The results of experiments with cytoplasmic calcium chelator BAPTA-AM and protein kinase inhibitor H7 indicate that the mechanism of protein synthesis synchronization in MSC consists in calcium-dependent phosphorylation of cell proteins. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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