| Autor: |
R, Fiorini, G, Curatola, A, Kantar, P L, Giorgi, E, Bertoli, F, Tanfani |
| Rok vydání: |
1990 |
| Předmět: |
|
| Zdroj: |
Biochemistry international. 20(4) |
| ISSN: |
0158-5231 |
| Popis: |
Human erythrocytes were separated using Percoll/Albumin density gradient centrifugation into 5 subpopulations which represent cell subpopulations of different ages. The physico-chemical properties of the membranes and their lipid extracts, prepared from the 5 subpopulations, were studied by 1,6-diphenyl-1,3,5-hexatriene fluorescence polarization and by Fourier Transform Infrared Spectroscopy respectively. The fluorescence polarization data show a decrease of membrane fluidity in the youngest erythrocyte fraction and the infrared spectroscopy reveals a lower degree of unsaturation of the lipids from this fraction. Changes of membrane fluidity among the 5 erythrocyte subpopulations is assigned to the variations of the degree of unsaturations of lipids rather than to variations of cholesterol content. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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