| Autor: |
Hugo, Barcenilla, Mikael, Pihl, Florence, Sjögren, Louise, Magnusson, Rosaura, Casas |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2559 |
| ISSN: |
1940-6029 |
| Popis: |
Regulatory T cells are an important component of the immune system that plays a key role in maintaining homeostasis. Identification of distinct regulatory T cell subsets is essential to understand their function. Mass cytometry or CyTOF is a technology that enables the simultaneous measurement of up to 50 markers in single cells by using antibodies tagged with heavy metals, which are then detected with time-of-flight mass spectrometry. This chapter describes a mass cytometry approach for phenotypic characterization of regulatory T cells and determination of their master transcription factor Foxp3. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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