| Autor: |
Clémentine, Brocas, Cécile, Ducrot, Karine, Dubrana |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2004 |
| ISSN: |
1940-6029 |
| Popis: |
The cohesin complex is required to establish sister chromatid cohesion and ensure accurate chromosome segregation after DNA replication. Recent data has also revealed a role for cohesion as a major player in DNA repair and gene expression regulation. All subunits of the Cohesin complex are essential and cannot be deleted. Here, we describe a protocol to efficiently deplete cohesin subunits with an auxin-inducible degron (AID) system in S. cerevisiae. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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