Enhanced production and characterization of a highly stable extracellular protease from an extreme halophilic isolate Salicola marasensis
| Autor: | Khoshnevis, N., Shahla Rezaei, Samaei-Nouroozi, A., Amin, M., Moshfegh, M., Khoshayand, M. R., Faramarzi, M. A. |
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| Zdroj: | Scopus-Elsevier Iranian Journal of Pharmaceutical Research : IJPR |
| Popis: | Owing to their superior catalytic activity in the extreme conditions, extremozymes have found the potential biotechnological applications for industrial purposes. A robust extracellular protease activity was detected in the culture broth of Salicola marasensis, an extreme halophilic bacterium, after a 48 h-incubation. The effect of different media ingredients in a liquid state fermentation was followed with the aim of improving the enzyme production yield. Fractional factorial and Box-Behnken designs were applied to get a 3.4 fold (from 6.0 to 20.3 U mL−1) improvement of protease production. The distinguishing features of this enzyme were stability at a wide range of pH (5.0–11.0) and temperature (25–60 °C), significant compatibility towards organic solvents, metal ions, chemicals, and surfactants, and hydrolysis of a variety of substrates. The properties of this enzyme can be of tremendous help in terms of the halophilic proteolytic extract’s industrial applications. |
| Databáze: | OpenAIRE |
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