| Autor: |
Vierra, M., Garachtchenko, T., Gupta, V., Schmid, I., Hawley, T., Cimbro, R., Farmer, A., Haugwitz, M. |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Popis: |
Analysis of cells via flow cytometry requires calibration of the instrument with the fluorophore used to label the cells of interest. Commercially-available calibration beads are an indispensable tool when preparing flow cytometers for experiments using fluorescent dyes (e.g. FITC); however, due to the different spectral characteristics of fluorescent proteins versus fluorescent dyes, existing fluorescent dye beads are not suitable for instrument calibration if the cells being analyzed express fluorescent proteins. Therefore, we developed calibration beads labeled with either the red fluorescent protein mCherry or the green fluorescent protein AcGFP, which has spectral properties almost identical to EGFP. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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