Evaluating Effects of Cell Sorting on Cellular Integrity

Autor: Lopez, P., Bergeron, A., Box, A., Brundage, K., Chittur, S., Cochran, M., Meyer, E. M., Tighe, S., DeLay, M.
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Popis: During the past year the Flow Cytometry Research Group has continued on its goal to establish best practice guidelines for cell sorting conditions that minimize cell stress, perturbation, or injury to the sorted cells. Towards this goal the group has followed up on an observation from our initial study that showed poor cell recovery when a clonal population of cells (Jurkat) was sorted aggressively under intentionally adverse sorting conditions (excessive pressure as well as undersized sorting orifice). In this follow-up study we sought to identify unique qualities of the cells that survived the adverse sorting conditions, in the hope that this may prove to be a useful test method for assessing deleterious effects of cell sorting across a wide variety of cell types.
Databáze: OpenAIRE