Utilizing the Rheonix NGS OnePrep™ Solution to automate the Takara Bio ThruPLEX® Tag-Seq HV library preparation kit

Autor: Higgins, Hazel, Zhu, Hui, Kim, Beum Jun, Yasmin, Rubina, Sherlin, Luke, Laliberte, Julie, Martin, Patrick, Farmer, Andrew, Montagna, Richard
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: J Biomol Tech
Popis: The fully automated Rheonix NGS OnePrep solution streamlined NGS library preparation for the new Takara Bio ThruPLEX Tag-Seq HV kit for Pan-Cancer targeted sequencing. Molecularly tagged, sample-indexed, sequence-ready libraries were produced with the Encompass Optimum workstation and microfluidic Rheonix CARDA cartridge. Data demonstrated that automated and benchtop prepared libraries were equivalent and allowed the detection of low (1%) allele frequency variants. The workflow significantly reduces hands-on time and total time-to-results while increasing sample throughput.
Databáze: OpenAIRE