| Autor: |
Lueking, A., Gutjahr, C., Gruß, V., Schulte, K., Müllner, S., Garretson, R., Beator, J. |
| Jazyk: |
angličtina |
| Rok vydání: |
2007 |
| Předmět: |
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| Popis: |
Protein Microarrays enable the analysis of multiple binding events in parallel. Based on patented UNIclone technology, we have established a reproducible protein biochip platform to determine specific binding profiles to multiple targets in a quantitative way. The UNIchip protein biochips provide a novel and unique tool for antibody characterization and ranking. Potential off-target activities can be detected by establishing a quantitative binding profile of an antibody to 400 different, unrelated targets. Together with on-chip determination of sensitivity, linearity and dynamic range the quantitative fingerprint enables a unique performance ranking and specificity analysis of antibodies. We have analyzed the performance of three block-buster biotherapeutics directed against TNF-alpha. The biotherapeutics show a clear differentiation both with regard to their binding profile to unrelated proteins as well as their binding affinity to the cognate target on the biochip. The quantitative fingerprint also offers a novel strategy for antibody pair selection in ELISA development. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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