| Přispěvatelé: |
Vinci, Mc, Tessitore, G, Castiglioni, L, Soncini, M, Consolo, Filippo, Micheli, B, Prandi, F, Santoro, R, Sironi, L, Polvani, G, Pesce, M. |
| Popis: |
Background - The pericardium is a natural tissue used for derivation of bio-prosthetic cardiac valves. Common decellularization procedures are not optimized providing risks of post- implantation inflammatory reaction. Specific aims and Results - The aim of this work was to devise a procedure to decellularize the pericardium maintaining mechanical compliance with reduced risks for rejection into an immunocompetent environment, also after cryopreservation. Pericardium sheets from tissue donors were dissected and incubated into a hypotonic buffer containing DNAse I, removing all cellular remnants. The decellularized tissue, with or without 60 days cryopreservation in N2 vapor was used for mechanical testing and implanted into immunocompetent mice. Mechanical response was assessed by uniaxial stress/strain tests. These showed minimal changes in decellularized (DE) vs. decellularized/cryopreserved (DE/CR) tissues. In particular, the elastic modulus at low (0.3±0.16 vs. 0.5±0.3) and high (26.5±16.6 vs. 31.0±13.7 MPa) strains were similar, while stress at failure (0.4±0.2 vs. 0.47±0.2 MPa) and at transition (3.7±1.8 vs. 5.1±2.4 MPa) were only slightly higher (P |
