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Introduction: Origanumminutiflorum belongig to the Lamiaceae used as ‘Oregano’ in Turkey. O. minutiflorumis an endemic species that has been sustainably collected for years fromSütçüler, province of Isparta in Turkey. Although O. minutiflorum is amongthe commercial Oregano and is widely used with various nutrients in Turkey andin many exporting countries, there is not enough studies on its pharmacologicaleffects (1). LOX enzymes are associated with inflammation-related diseases, andneurodegenerative diseases (2). In this study is aimed to determination of in vitro lipoxygenase enzyme inhibitionof the aerial parts of O. minutiflorum.Materials and Methods: Plant material was collected fromSütçüler, Isparta, Turkey in 2017. Crude extract was prepared with 70% ethanoland was subjected to liquid-liquid extraction according to polarity of thefollowing solvents; n-hexane,dichloromethane, etylacetate and n-butanol,resprectively. Llipoxygenase (LOX) inhibition activity was madespectrophotometrically by modifying the method defined by Baylac and Racine (3).Nordihydroguyaretic acid (NDGA) was used as positive control. All measurementswere repeated three times and the results were expressed as Mean ± Standarddeviation.Results: It was determined that thedichloromethan extract (100 μg/mL) showed the highest inhibition on the LOXenzyme (82.33±0.94%), while % Inhibition of the other extracts (100 μg/mL),40.94±1.81% (ethyl acetate), 24.50±0.50% (n-buthanol) and 17.45±0.55% (hexan).No activity found in the residual extract. NDGA was determined as 99.00±0.00µg/mL.Conclusions: As far as we know, the present work is thefirst contribution into LOX enzyme inhibition activity of O. minutiflorum.AcknowledgementsWe aregrateful to Prof. Dr. Yavuz Bulent Kose, Faculty of Pharmacy, Department of PharmaceuticalBotany, Anadolu University, Eskişehir, Turkey, for determination of the plantspecimen. References: 1. Baser, K. H. C. (2002). The TurkishOriganum species. In: Oregano, The genera Origanum and Lippia, Ed.:S.E.Kintzios, Taylor and Francis, London, pp. 109-126.2. Sacan, O., Turhan, E. Y. (2014).Lipoxygenase inhibitory activities of some plant extracts and chemicalcompounds. European Journal of Biology, 73 (2), pp. 47-52.3. Baylac, S., Racine, P. (2003). Inhibition of5-lipoxygenase by essential oils and other natural fragrant extracts.International Journal of Aromatherapy, 13 (2-3), pp. 138-142. |
