| Popis: |
ABIOTIC STRESSFACTORS AND SIGNAL MOLECULES AFFECT SECONDARY METABOLITES COMPOSITON OF ST.JOHN’S WORT BOTH IN VIVO AND IN VITROİN VİVO VE İN VİTRO ŞARTLARDA YETİŞTİRİLEN SARIKANTARON BİTKİSİNDE ABİYOTİK STRES FAKTÖRLERİ VE SİNYAL MOLEKÜLLERİNİN SEKONDERMETABOLİT KOMPOZİSYONUNA ETKİLERİNeşe ERAY VURANRes. Asist., Van Yüzüncü Yıl University, Faculty of Science, Departmentof Molecular Biology and Genetic, neseeray@yyu.edu.tr (CorrespondingAuthor), Orcid ID: 0000-0001-6387-1493Abdullah DALARAssoc. Prof. Dr., Van Yüzüncü Yıl University, Faculty of Pharmacy, dalar.abdullah@yyu.edu.tr, OrcidID: 0000-0002-0080-2519Musa TÜRKERProf. Dr., Yildiz Technical University, Faculty ofEngineering, Department of Bioengineering, musaturker@yahoo.com,Orcid ID: 0000-0003-3195-1119Inthe present study, Hypericum perforatumL. was regenerated in vitro and abioticstress factors and signal molecules were applied on in vitro regenerants toinvestigate the change of phenolic compounds concentration comparing to plantscollected from field. The effects of media composition, explant type and PlantGrowth Regulators (PGRs) in different combination and concentration on callusproduction, plant regeneration and secondary metabolite production wereevaluated. The quantitative analysis of phenolic composition was performed byHigh Performance Liquid Chromatography (HPLC). Accordingto the results, MS medium supplemented with 1 mg/L 2, 4-D + 1 mg/L BA wasdetermined to be the best medium for callus formation in dark. When micro propagationis considered, MS medium supplemented with 2 mg/L NAA + 1 mg/L BA was the best PGRscombination. Multiple shoots developed in MS supplemented with 2 mg/L NAA + 1mg/L BA were separated from each other and rooted in hormone-free MS medium. Theleaf explant gave the highest shoot number. The shoot explant produced thehighest amount of callus. Theeffects of abiotic stress factors such as drought and UV-C and some signalmolecules such as ascorbic acid, salicylic acid, sucrose and different PGRs onthe production of phenolic compounds were investigated.It was demonstrated that callus was able to producehypericin and pseudohypericin compounds. B5 and SH media gave better resultsthan MS medium for the production of hypericin and pseudohypericin. Thecombination of NAA + BA was detected the best PGRs on phenolic compoundsproduction. Chlorogenic acid concentrationwas increased by UV and sucrose, quercetin was increased by ascorbic acid andpseudohypericin was increasedby drought applications compared to the control. The increases in the amounts of phenolic compounds werestatistically significant (p in vivo and in vitro. However, the plant grown inlaboratory conditions produced rutin compound under drought stress.Keywords:Abiotic stress, Hypericum perforatumL., Phenolic compound, Signal Molecule, Tissue culture |
