| Popis: |
R-limonene is a terpene derivative, which has antimicrobial activity. The aim of this studyis to evaluate the antibacterial efficacy of R-limonene nanoemulsions against Enterococcusfaecalis, which is highly resistant to repetitive applications of calcium hydroxide used in thechemomechanical preparation of root canal treatment. Oil/water R-limonenenanoemulsions were prepared by phase inversion method. Formulation factors are listedin the table below (Table 1). The particle size (PS), polydispersity index (PDI) and zetapotential (ZP) values were evaluated and were given in the table below (Table 2). Itwas observed that the ZP values decreased as the Tween 80 ratio increased. Therefore, F3,F4 and F6 formulations were eliminated. F1, F2 and F5 formulations were subjected to a oneweek stability test at 4, 25 and 40 °C and it was observed that the ZP of the F1 formulationdecreased at all temperatures. There were not significant changes in the F2 and F5formulations. The MIC values of F2 and F5 formulations were determined compared to pure Rlimonene on Enterococcus faecalis. The MIC values of pure R limonene, F2 and F5 were 210.3mg/mL, 49.80 mg/mL and 48.82 mg/mL, respectively. These results show that emulsionformulations of R-limonene are approximately 4.5 times more effective than pure R-limonene. |
