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In the last twenty years there has been increased usage of metil tertiary butil eter (MTBE) which led to it's high presence in the environment. Environmental Protection Agency (EPA) has characterized MTBE as potentially cancerogenic substance and proposed the methods for it's removal from natural sources. Bioremediation represents the world trend in developing remediation technologies, and is a complex process which assumes using different microorganisms. The etheric bond in MTBE's molecule is persistent in the environment. Still, microorganisms are capable for using this organic compaund as carbon and energy source due to their various enzyme systems and transform it into non toxic products. The objective of this research is investigation of biodegradability of various concentrations of MTBE (76, 100 438 and 746 ppm) by Mucor hiemalis 139ž. This strain has been isolated from the soil of NIS Oil Raffinery Pančevo, contaminated with organic pollutants. The degradation process was observed during 8 days of incubation in aerobic conditions. The MTBE’s degradation was detected by gas chromatography with flame ionizing detector (GC/FID). After 12 hours of incubation the concentration of MTBE was reduced. The MTBE’s reduction ranging form 47 to 76% from the initial concentration was detected after 8 days. The degradation level depended on the starting concentration of MTBE. These results indicate the possibility for using the authentic strain Mucor hiemalis in the bioremediation processes of soils and waters, as ecologically, economically and energetically sustainable remediation methods. Poslednjih 20 godina intenzivna upotreba metil tercijarnog butil etra (MTBE) dovela je do povećanja njegovog prisustva u čovekovoj sredini. Agencija za zaštitu čovekove sredine (EPA) okarakterisala je MTBE kao potencijalno kancerogeno jedinjenje i predložila metode za njegovo uklanjanje iz prirodnih sredina. Bioremedijacija predstavlja svetski trend u razvoju tehnologija remedijacije i sastoji se od kompleksa procesa koji podrazumevaju korišćenje različitih vrsta mikroorganizama. Mikroorganizmi su, zahvaljujući svojim fermentnim sistemima, sposobni da koriste različita organska jedinjenja kao jedinstven izvor ugljenika i energije i transformišu ih do netoksičnih produkata. Cilj rada bio je ispitivanje degradacije različitih koncentracija MTBE-a (76, 100, 438 i 746 ppm) pomoću Mucor hiemalis 139ž. Ovaj izolat izolovan je iz zemljišta NIS Rafinerije nafte Pančevo, kontaminiranog organskim zagađivačima. Proces degradacije praćen je u toku 8 dana inkubacije pri aerobnim uslovima. Degradacija MTBE-a praćena je pomoću gasnog hromatografa sa plamen jonizujućim detektorom (GC/FID). Posle 12 sati inkubacije došlo je do smanjenja koncentracije MTBE-a. Stepen degradacije MTBE-a posle 8 dana se kretao u intervalu od 47-76% od početne koncentracije. Stepen degradacije zavisio je od početne koncentracije MTBE-a. Ovi rezultati ukazuju na mogućnost primene autohtonog izolata Mucor hiemalis 139ž u procesima bioremedijacije zemljišta i voda, kao ekološki, ekonomski i energetski prihvatljiv metod remedijacije. |
