Toxicity effect of silver nanoparticles on photosynthetic pigment content, growth, ROS production and ultrastructural changes of microalgae chlorella vulgaris

Autor: Hazeem, L.J., Kuku, G., Dewailly, E., Slomianny, C., Barras, A., Hamdi, A., Bououdina, M.
Přispěvatelé: Hazeem, L.J., Kuku, G., Dewailly, E., Slomianny, C., Barras, A., Hamdi, A., Bououdina, M., Yeditepe Üniversitesi
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Popis: Silver nanoparticles (Ag NPs) exhibit antibacterial activity and are extensively used in numerous applications. The aim of this study was to examine the toxic effect of Ag NPs on the marine microalga, Chlorella vulgaris. The microalgae, at the exponential growth phase, were treated with different concentrations of Ag NPs (50 and 100 nm) for 96 h. X-Ray diffraction (XRD) results indicated that the used NPs are single and pure Ag phase with a mean crystallite size of 21 and 32 nm. Ag NPs were found to have a negative effect on viable cell concentration, a variable effect on chlorophyll a concentration, and increased ROS formation. Transmission electron microscopy (TEM) analysis revealed that Ag NPs were present inside the microalgae cells and formed large aggregates in the culture medium. Ag+ ions, in the form of AgNO3, were also assessed at higher concentrations and found to cause inhibitory effects. © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Lillehei Heart Institute, University of Minnesota Deanship of Scientific Research, King Saud University Centre National de la Recherche Scientifique University of Bahrain Funding: This work was supported by grant number (11/2012) funded by Deanship of Scientific Research, University of Bahrain. A.B., A.H., and R.B. thank the Centre National de la Recherche Scientifique (CNRS), the University of Lille, and the Hauts-de-France region for their financial support. G.K. and M.C. thank Yeditepe University for their financial support.
Databáze: OpenAIRE