| Autor: |
Ozgenturk, N.O., Ulu, Z.O., Ulu, S., Dogan, S., Tuna, B.G. |
| Přispěvatelé: |
Ozgenturk, N.O., Ulu, Z.O., Ulu, S., Dogan, S., Tuna, B.G., Yeditepe Üniversitesi |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| Popis: |
Introduction: In the present study, RNA sequencing-mediated transcriptome analysis was performed in order to elucidate the molecular mechanisms of the immune response for different types of calorie restriction (CR) application using MMTV-TGF-? breast cancer mouse model. Methods: Animals were applied to three different dietary regiments; ad libitum (AL), chronic calorie restriction (CCR) and intermittent calorie restriction (ICR). Using thymus tissues, 6091 differentially expressed genes (DEGs) were identified in three dietary groups. After clustering of total of 6091 DEGs using Gene Ontology (GO) categories, a total of 400 genes were identified to be involved in immune system process (GO:0002376) GO categories. KEGG pathway and gene co-expression network analysis of these immune-related DEGs were done using String database. The results were confirmed with measuring mRNA expression levels of four selected immune-related DEGs genes (Casp3, Thy1, IL-16 and CD4) using quantitative real-time PCR (qPCR). Results: The expression levels of immune-related genes were different in three RNA-seq data. Conclusion: The results provide useful information to investigate the immune-related transcriptional profiling in thymus tissue of breast cancer mouse model applied to two different types of CR and to identify the specific functional immune related genes in response to CR during cancer development. © 2019 De Gruyter. All rights reserved. 2016-01-07-DOP01 114S429 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu Acknowledgments: This work was supported by Yildiz Technical University Scientific Research Projects Coordinator (Grant 2016-01-07-DOP01). The animal experiments were supported by The Scientific and Technological Research Council of Turkey (TUBITAK 114S429). The authors thank Dr. Margot P. Cleary for generously donating the breeding colony of the MMTV-TGF-? transgenic mice. The authors thank Munevver Burcu Cicek-dal, Ilker Coban, Busra Kazan, and Mustafa Erhan Ozer for overseeing the breeding and genotyping protocols to obtain the experimental animals. The authors also thank the veterinarian and animal technicians who handle the animals at Yeditepe University Animal Facility (YUDETAM). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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