| Autor: |
Dundar, N.O., Aktekin, B., Ekinci, N.C., Sahinturk, D., Yavuzer, U., Yegin, O., Haspolat, S. |
| Přispěvatelé: |
Dundar, N.O., Aktekin, B., Ekinci, N.C., Sahinturk, D., Yavuzer, U., Yegin, O., Haspolat, S., Yeditepe Üniversitesi |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
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| Popis: |
Mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS) is a common medically intractable epilepsy syndrome. Although pathogenesis of HS still remains highly controversial, genetics may play a role as a predisposing factor. Previous evidence in a Japanese population revealed that the homozygotes for allele T at position -511 of the interleukin (IL)-1ß gene promoter region (IL- 1ß-511 T/T) confers susceptibility to the development of HS. However, whether this polymorphism has an effect on IL-1ß levels in MTLEHS patients was not demonstrated. This study aimed to analyze the distribution of this particular polymorphism in a group of Turkish HS patients and correlate the polymorphism with IL-1ß secretion from the lymphocytes, thus revealing a functional role for IL-1ß in the etiopathogenesis of HS. A single base pair polymorphism at position -511 in the promoter region of the IL-1ß gene was analyzed. The spontaneous and 1 ng/mL lipopolysaccharidestimulated production of IL-1ß by peripheral blood mononuclear cells after 4 and 24 h of incubation were measured by ELISA method. The heterozygous type (-511 C/T) was the most common genotype. There was no difference in frequency of allele -511 T between patients and controls. Analysis of IL-1ß levels, genotype and allele distributions showed no significant difference among the groups (P>0.05). Nevertheless, it was seen that patients who carry a T allele at position -511 of the IL-1ß gene had increased IL-1ß levels. Tallele carriage may be important. Only IL-1ß secretion from the lymphocytes has been assessed in this study. Considering the importance of IL-1ß in the etiopathogenesis of HS, further studies are needed to evaluate locally produced IL-1ß levels. © N.O. Dundar et al., 2013 Licensee PAGEPress, Italy. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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