| Autor: |
Dimitrova, Ivona, Bozhilova-Sakova, Milena, Ignatova, Maya, Ivanova, Tanya, Koutev, Veselin, Iliev, Margarit |
| Rok vydání: |
2020 |
| Předmět: |
|
| Popis: |
GDF9 (growth differentiating factor 9) gene is a candidate marker affecting prolificacy. The aim of this study was to investigate the genetic variability in locus SNP G1 of the GDF9 gene in three merino (Ascanian, Caucasian and Karnobat) sheep breeds in Bulgaria. A total of 90 ewes, 30 of each breed were investigated for polymorphisms in SNP G1 of the GDF9 gene. A 462 bp fragment from GDF9 sheep gene was amplified using the PCR technique. The genotypes of all animals were established using PCR-RFLP analysis with restriction enzyme HhaI. This is the first study of GDF9 in Bulgarian sheep breeds and satisfactory diversity was found. The frequency of allele A was lower and predominance of allele G was found. All possible genotypes (GG, AG and AA) were identified only in Karnobat merino breed. In the other two breeds Ascanian and Caucasian, genotypes GG and AG were observed, while the mutant homozygous genotype AA was not detected. The frequencies of the wild allele G were significantly higher than those of mutant allele A in all studied breeds. The heterozygous genotype AG was with the highest frequency (0.27) in Karnobat merino breed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
