| Autor: |
Bozhilova-Sakova, Milena, Dimitrova, Ivona, Ignatova, Maya, Teneva, Atanaska, Ivanova, Tanya, Koutev, Vesselin, Stoikova-Grigorova, Radostina, Viryanski, Denis |
| Rok vydání: |
2022 |
| Popis: |
The success of sheep production depends largely on two important characteristics of sheep – their growth ability and meat quality. The myostatin gene (MSTN) is one of the major regulators of skeletal muscle growth and development and it is defined as a suitable candidate gene for genetic improvement through marker-assisted selection. In this study by means of PCR-RFLP method was identified allelic variants of intron 1 of MSTN gene in ten Bulgarian sheep breeds – Askanian (30 animals), Caucasian (30 animals), Karnobat Merino (60), Local Karnobat (30), Karakachan (60), Breznik (30), Cooper- Red Shoumen (30), Il de France (60), Bulgarian Dairy Synthetic Population (60) and Pleven Black headed (30). Genomic DNA was extracted from 420 blood samples. After PCR amplification, the received 497 bp fragment of the myostatin gene was digested with the restriction enzyme DraI. The results showed that this area of the myostatin gene is highly conservative in Bulgarian sheep breeds. All of the investigated ten sheep breeds were monomorphic for the tested locus. Only the B allele and genotype BB were found in all studied animals. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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