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WOS: 000221704400003 The aims of this study were to determine the alpha naphthyl acetate esterase (ANAE) activity in peripheral blood leukocytes and the ratio of T lymphocytes in healthy adult Ostriches. Blood smears from 20 adult and healthy Ostriches were tested for detecting ANAE activity by a specific staining procedure. Thrombocytes, heterophils and eosinophile granulocytes gave negative reaction, whereas monocytes showed a diffuse positivity. On the contrary, the pattern of ANAE in T lymphocytes was granular, with 1 or 2, sometimes more, red granules. In blood, the percent of ANAE positive lymphocytes was 59.3 +/- 0.40%. In conclusion, ANAE activity showed a specific granular expression in T lymphocytes in Ostriches and allowed to determine directly and quickly the ratio of circulationg T lymphocytes. |
