Involvement of the histaminergic system in the resuscitating effect of centrally acting leptin in haemorrhagic shock in rats
| Autor: | Jochem, Jerzy, Ottani, Alessandra, Giuliani, Daniela, Kasperska-Zajac, Alicja, Guarini, Salvatore |
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| Přispěvatelé: | Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Farmakoloji Anabilim Dalı., Altınbaş, Burçin, Yalçın, Murat, Savcı, Vahide, AAG-6956-2021 |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Leptin
Male Rats wistar Histamine H3 receptor antagonist Unclassified drug Physiology Microdialysis Histaminergic system Wistar rat Procedures Histamine H4 Receptors Thioperamide Intracerebroventricular Drug Administration Tachycardia Mechanisms Responses Intracerebroventricular drug administration 1 (5 chloro 2 benzimidazolylcarbonyl) 4 methylpiperazine Neurons Histaminergic receptor affecting agent Blood flow Nerve cell Mean arterial pressure Cardiovascular system Hemorrhagic shock Blood pressure Peripheral ischemia Histamine receptor antagonist Adrenergic system Injections intraventricular Hypotension Vasopressin Receptor Histamine Chlorpheniramine Vuf 5681 Heart rate Ischemia/reperfusion Ranitidine Pathophysiology Article Shock hemorrhagic Proopiomelanocortin Histamine agents Animals Animal model Benzimidazole derivative Animal experiment Kidney blood flow Histamine receptor Drug effects Haemorrhagic shock Animal Arterial-pressure Induced reversal Nonhuman Rats Release Sympathetic nervous system Regional blood flow Mesentery blood flow Rat Benzimidazoles Controlled study High performance liquid chromatography |
| Popis: | Leptin, acting centrally as a neuromodulator, induces the activation of the sympathetic nervous system, which may lead to a pressor action in normotensive animals. In haemorrhagic shock, leptin administered intracerebroventricularly (icv.) evokes the resuscitating effect, with long-lasting rises in mean arterial pressure (MAP) and heart rate (HR), subsequent increase in peripheral blood flows, and a 100% survival at 2 h. Since leptin is able to activate histaminergic neurons, and centrally acting histamine also induces the resuscitating effect with the activation of the sympathetic nervous system, in the present study, we investigated an involvement of the histaminergic system in leptin-evoked cardiovascular effects in haemorrhagic shock. The model of irreversible haemorrhagic shock, with MAP decreased to and stabilised at 20 - 25 mmHg, has been used. Leptin (20 mu g) given icv. at 5 min of critical hypotension evoked 181.5% increase in extracellular hypothalamic histamine concentration during the first 10 min after injection. Rises in MAP, HR and renal, mesenteric and hindquarters blood flows induced by leptin were inhibited by icv. pre-treatment with histamine H-1 receptor antagonist chlorpheniramine (50 nmol). In contrast, there was no effect of H-2, H-3 and H-4 receptor antagonists ranitidine (25 nmol), VUF 5681 (25 nmol) and JNJ 10191584 (25 nmol), respectively. In conclusion, the histaminergic system is involved in centrally-acting leptin-induced resuscitating effect in haemorrhagic shock in rats. Medical University of Silesia, Katowice, Poland - KNW-1-065/P/2/0 European Cooperation in Science and Technology (COST) - BM0806 |
| Databáze: | OpenAIRE |
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