Sublethal toxicity of esbiothrin relationship with total antioxidant status and in vivo genotoxicity assessment in fish (Cyprinus carpio L., 1758) using the micronucleus test and comet assay
| Autor: | Selvi, Mahmut, Benli, A. Çağlan Karasu, Memmi, Burcu Koçak, Dinçel, Aylin Sepici, Sarıkaya, Rabia, Erkoç, Figen Ünlü |
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| Přispěvatelé: | Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü., Çavaş, Tolga, Vatan, Özgür, Çinkılıç, Nilüfer, Yılmaz, Dilek, Zorlu, Tolga, AAH-3508-2021, O-7508-2015, AAH-5296-2021 |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Water resources
Insecticides Erythrocytes Dose-response relationship Unclassified drug Micronucleus tests Cytotoxicity Hemoconcentration LC 50 Peripheral erythrocytes Aquatic ecosystem Toxicology Ecotoxicology Antioxidants Induced oxidative stress Coprosma kauensis Osmoregulation Total antioxidant status Behavior change Allethrin Responses Enzyme activity Pyrethroid insecticides Insecticide Priority journal Hexapoda Cyprinid Nontarget organism Ames test Erythrocyte Blood Hematocrit Carp Pisces Comet Assay Micronucleus Tests Biomarkers Antioxidant Sublethal effect Mosquito repellents Carps Pollution exposure Cyprinidae Liza-saliens Health status indicator Article Exposure Dna-damage Database Esbiothrin Water fish Cyprinus carpio Antioxidant activity Environmental sciences & ecology Micronucleus test Animals Animal model Animal experiment Comet assay Toxicity test Channa-punctata bloch Capacity Toxicity Hematopoietic tissue Comet assays Concentration (composition) Nonhuman Mesylic acid ethyl ester Environmental sciences Fish Erythroid cell Micronucleus Oxidative stress DNA damage Genotoxicity Controlled study |
| Popis: | Esbiothrin, synthetic pyrethroid with quick activity against insects, is widely used against household pests and in public health. Despite widespread use, data on ecotoxicity and genotoxic effects are extremely scarce. The aim of the present study is to evaluate the genotoxic potential of esbiothrin on a model fish species Cyprinus carpio L., 1758 (Pisces: Cyprinidae, koi) using the micronucleus test and comet assay in peripheral blood erythrocytes. Effects of two sublethal exposure concentrations on plasma total antioxidant status (TAS mmol/L), and Hct values were examined. On the basis of the 96 h LC50 data from U.S. EPA ecotox database (32 g/L) two sublethal exposure concentrations (5 and 10 g/L) were used together with ethyl methanesulfonate (EMS) (5 mg/L) as positive control. Five fish were used for each dose/duration group (24, 48, and 72 h) under controlled laboratory conditions. The fish showed behavioral changes at the higher dose. Plasma TAS (mmol/L) levels decreased in 24 h; an increase was observed slightly for 48 and obviously for 72 h in both exposure doses. Similarly, hematocrit (Hct) values differed between exposure duration but no significant differences in mean values were found between groups of the same exposure time. The general trend was a rise after 48 h, which decreased afterwards. Our results revealed significant increases in the frequencies of micronuclei and levels of DNA strand breaks and thus demonstrated the genotoxic potential of this pesticide on fish, a nontarget organism of the aquatic ecosystem. To our knowledge this is the first study to report observable genotoxic effects of esbiothrin on fish. Gazi Üniversitesi (BAP 18/2009-02) |
| Databáze: | OpenAIRE |
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