Effects of adrenomedullin and glucagon-like peptide on distal flap necrosis and vascularity: The role of receptor systems and nitric oxide
| Přispěvatelé: | Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Deney Hayvanları Yetiştirme ve Araştırma Merkezi., Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Biyofizik Anabilim Dalı., Cam, Betül, Bağdaş, Deniz, Özyiğit, Musa Özgür, Sağdilek, Engin, Büyükcoşkun, Naciye İsbil, Özlük, Kasım, AAH-1692-2021, AAH-2873-2021, AAR-6478-2021, AAH-4397-2021 |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Rats
wistar Glucagon-like peptides Survival Physiology Glucagon like peptide receptor Apoptosis Injury Wistar rat Disease models animal Antioxidants Adrenomedullin Endothelial growth-factor Pathology Calcitonin gene related peptide receptor Cell-proliferation Graft survival Flap necrosis Immunohistochemistry Glucagon like peptide Receptors calcitonin gene-related peptide Flaps (Control Surfaces) Perforator Flap Reperfusion Injury Calcitonin gene-related peptide A signaling pathway Rat model Female Antioxidant Skin flaps Glucagon-like peptide-1 Beta-cells Wound healing Dermatology Necrosis Animals Glucagon-like peptide receptors Animal Disease model Wounds and injuries Vascularization Epigastric arteries Nitric oxide Protein-kinase Rats Drug effect Epigastric artery Metabolism Surgical flaps Rat Surgery Angiogenesis Calcitonin gene related peptide |
| Popis: | Objective. Flap necrosis in the distal area due to the deficiency of blood circulation is a major complication in flap treatment. In many previous studies, some natural substances such as chlorogenic acid, adrenomedullin (ADM), and glucagon-like peptide-1 (GLP-1) have been used to improve flap viability via their vasodilator, angiogenic, and antioxidant effects. The aim of this study is to clarify the mechanism through the use of selective antagonists for calcitonin gene-related peptide (CGRP) receptors and GLP-1,receptors such as CGRP-(8-37), exendin-(9-39), respectively, in the flap healing effects of ADM and GLP-1. The role of nitric oxide (NO) was investigated in the mechanism as well. Materials and Methods. Seventy adult female Wistar rats (200 g-250 g) were used in the study. The cutaneous skin flap (8 cm x 3 cm) on the abdominal wall was raised based on the superficial inferior epigastric artery (SIEA). Single-dose substance injections were administered into the SIEA. Necrosis in the flap area was evaluated on postoperative day 7. The proportion of the necrosis area (necrosis area % = [necrosis area/ flap area] x 100) and vascularity (vascular number/cm(2)) in the distal area were calculated. Results. The administrations of ADM or GLP-1 increased the vascularity and decreased the necrosis area in the distal flap region. The ADM receptor antagonist, CGRP-(8-37), did not prevent the positive effects of ADM on flap healing and vascularity. A GLP-1 receptor antagonist, exendin-(9-39), prevented the effect of GLP-1 on flap healing and vascularity. Nitric oxide mediated the beneficial effects of both peptides on flap healing. Conclusion. The CGRP receptors have no direct role, but NO acts as a mediator in the beneficial effect of ADM on flap healing. The GLP-1 specific receptors and NO act as important interagents for the effects of GLP-1 on flap healing. |
| Databáze: | OpenAIRE |
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