| Popis: |
OBJECTIVES: Theobjektive of this study was to evaluated the differantitaion of humanadipose tissue derived mesenchymal stem cells (MSCs) into skeletal muscle cellsby the effect of IGF1 and FGF2 growth factors. METHODS: MSCswere divided into four groups. I.group (control group) was incubated only inmyogenic medium, II. group was incubatedby adding 100 ng/ml FGF2 to the myogenic medium, III. group was incubated byadding 20 ng/ml IGF1 to the myogenic medium, IV. group was incubated by adding100 ng/ml FGF2 and 20 ng/ml IGF1 to the myogenic medium. Cells dyed with MyoD1antibody were analyzed by flow cytometry so as to determine myogenicdifferentiation at the end of 28 days.RESULTS: Itwas confirmed that 11.5% of the control group, 39.2% of the FGF2 group, 23.1%of the IGF1 group and 39.3% of the IGF1-FGF2 group showed differentiation. CONCLUSION: Ourresults show that IGF1 and FGF2 have a positive contribution on myogenicdifferentiation of MSCs, contribute to the studies related to muscle diseasesand treatment of these diseases to support by growth factors the feature of theregeneration capacity and differentiation. KEYWORDS: Stem Cell, MyogenicDifferentiation, IGF, FGF |
