Nucleic Acid and Peptide Aptamers as Potential Antiviral Drugs
| Autor: | Evran S., Uğurlu Ö., Man E., Gültan M., Özyurt C. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
antivirus agent
viral protein Hepatitis B virus Antiviral aptamer SARS coronavirus Peptide aptamer polymerase chain reaction virus culture Ribosome display RNA directed DNA polymerase Herpes simplex virus MRNA display Article systematic evolution of ligands by exponential enrichment aptamer technique Wart virus antigen antibody core protein Severe acute respiratory syndrome coronavirus 2 Diagnostic aptamers viral nonstructural protein Therapeutic aptamers viral structural protein Arbovirus nonhuman SELEX messenger RNA Hepatitis C virus Human immunodeficiency virus reverse transcription loop mediated isothermal amplification virus neutralization test Aptasensor DNA aptamer Ebolavirus enzyme immunoassay real time reverse transcription polymerase chain reaction nucleic acid RNA aptamer ribosome colorimetry in vitro selection integrase Influenza virus |
| Popis: | Aptamers with target-specific binding properties have emerged as an alternative to antibodies. Nucleic acid aptamers are short single-stranded oligonucleotides that can fold into unique three-dimensional structures. Nucleic acid aptamers are selected from random libraries in vitro by using the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) technology. Likewise, peptide aptamers are short peptides that can be selected in vitro by using different strategies including phage display, ribosome display, or mRNA display. Aptamers are superior to antibodies with regard to ease of production, high stability, small size, and low cost. Therefore, aptamers find broad use in different biotechnological and therapeutic applications. Among them, aptamer use in virus detection and antiviral therapy is one of the attractive applications. The present Covid-19 pandemic and life-threatening viral infections reveal the need for rapid therapeutic solutions that can efficiently target viral mechanisms. In this respect, the chapter is mainly focused on aptamers with antiviral activity, as well as the use of aptamers in viral detection platforms. First, we summarize aptamer selection technologies that can be performed in vitro. Among them, we briefly explain ribosome display, mRNA display and SELEX (Systematic Evolution of Ligands by Exponential Enrichment) technologies. Then, we review aptamers targeting viral proteins and viral invasion mechanisms. In addition, we give an overview of aptamers developed against viruses. We also discuss the major hurdles in aptamer use, as well as the strategies to improve the drug potential of aptamers. © 2021 Bentham Science Publishers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|