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The aim of this study was to determine the effects of dietary sepiolite on performance, egg quality, egg yolk cholesterol content and some blood parameters of laying hens during 22 weeks period. For this purpose a total of 135 Lohmann Brown laying hens, 38 weeks of age, were allocated to one control group and two treatment groups. Sepiolite was used at the level of 0, 0.5 and 1% for the diets of control group and the first and second treatment groups, respectively. Dietary sepiolite supplementation did not significantly affect feed intake, egg production, egg weight and feed conversion ratio. The values of egg breaking strength (p |