Immobilization and characterization of β-glucosidase from gemlik olive (Olea europea L.) responsible for hydrolization of oleuropein

Autor:	Onat, Serhat, Savaş, Elif
Přispěvatelé:	Mühendislik Fakültesi
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Olea Europea L pNPG Beta-glucosidase Purification SPMN
Popis:	The beta-Glucosidase (beta-D-glucoside glucohydrolase, EC 3.2.1.21) enzyme was purified from Gemlik variety olive (Olea europea L.). The purified enzyme was immobilised onto Supermagnetic Nanoparticles in order to stabilise the enzymatic efficiency and increase usage in the food industry. The purified and immobilised enzyme was characterised by molecular weight, kinetic parameters and optimum pH and temperature values comparatively. The enzyme is a monomer with a mass of approximately 40 kDa. The Kinetic values of the immobilised and purified enzymes were 1.34 mM and 384.61 U/mg; 0.37 mM as Km and 370.37 U/mg as Vmax respectively. Research Fund of the University of Balikesir BAP.3.2016.0001 Turkish Research Council, Turkey 110O778, PT2013-01335
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=od______4609::67cec6302d985e831ea07369bee43502 https://hdl.handle.net/20.500.12462/10937 Zobrazit plný text záznamu