| Popis: |
In this study, it was aimed to determine the influence of L-tryptophan supplementation of the extender on cryopreservation of Tigris scraper (Capoeta umbla) spermatozoa. The standard extender was supplemented with 0 mM, 0.5 mM, 1 mM, 2 mM and 4 mM L-tryptophan. Sperm samples diluted at a ratio of 1:3 with the extenders were subjected to cryopreservation. After dilution the sperm was aspirated into 0.5 ml straws, placed on a tray to freeze in nitrogen vapor and then, plunged into liquid nitrogen. L-tryptophan positively affected post-thaw sperm motility, and L-tryptophan could be added to extenders. Comparing all concentrations of L-tryptophan, the best concentration of L-tryptophan was 1 mM. |
