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Strain Streptomyces (S.) hygroscopicus CH-7 during the fermentation of polyketide antibiotic produces as a by product proteolytic complex with significant trypsin like activity. Also, S. hygroscopicus CH-7 produces extracellulary proteinaceous protease inhibitor known as Streptomyces subtilisine like inhibitor (SSL-inhibitor). Detection of SSL-inhibitor is preformed on the base of stability of SSL-inhibitors on very low pH (final concentracion of TCA is 9%). Molecular mass of SSL-inhibitor is approximately 11 kDa, and it provides very wide range of inhibitory activity toward different serin proteases: trypsin, protease from Bacillus (B.) subtilis, proteinase K from Tritirachium (T.) album, protease from B. licheniformis, protease from S. griseus and the complex of proteases from S. hygroscopicus CH-7. Soj S. hygroscopicus CH-7 u toku fermentacije ekstracelularno proizvodi proteinski inhibitor proteaza koji pripada grupi Streptomyces subtilizinu sličnim inhibitorima (Streptomyces subtilisine like inhibitor: SSL-inhibitor). Postupak za detekciju je jednostavan i bazira se na činjenici da su SSL-inhibitori stabilni na veoma niskim pH. SSL-inhibitor iz S. hygroscopicus CH-7 pokazuje širok spektar inhibitornog dejstva na: tripsin, proteazu iz B. subtilis, proteinazu K iz T. album, proteazu iz B. licheniformis, proteazu iz S. griseus i na sirovi proteoliticki kompleks S. hygroscopicus CH-7. Molekulska masa SSL-inhibitora određena gel filtracijom iznosi 11 kDa, slično većini do sada izolovanih SSL. |
