| Popis: |
Selenium is a trace element, which stimulates antioxidant defenses and improves reproductive functions in human and animals, under the form of selenoproteins. The objective of the study was to evaluate the effect of selenium, supplemented as inorganic or organic form in the diet of stud rams, on some of their semen parameters. The experiment was performed with 15 clinically healthy rams from North East Bulgarian merino breed. The animals were divided in three groups (5 per group). The rams from first experimental group (G1) received a diet with supplementation of 4,0mg sodium selenite (Na2SeO3) per animal per day, while the animals of the second experimental group (G2) obtained diet with 1.83g L-selenomethionine (Sel-Plex, Alltech, USA) per animal per day. Eventually, each animal from the G1 and G2 received 1.83g selenium per day. The control group (GC) received a diet without supplementation of selenium. The principal composition of the diet in each group was the same. The ejaculates were obtained via artificial vagina. The evaluated parameters were volume and pH of the ejaculates and motility, concentration and in vitro survivability of the spermatozoa at 39˚S for 360 min. It was found that the supplementation of ram studs diet either with inorganic and organic selenium led to increase in the volume of the ejaculates, motility and survivability of the spermatozoa. The pH of the freshly obtained semen was not affected by selenium treatment. Selen je element u tragovima, koji stimuliše antioksidantske odbrane i poboljšava reproduktivne funkcije kod ljudi i životinja, u obliku selenoproteina. Cilj studije je bio da se proceni efekat selena, dopunjenog u neorganskom ili organskom oblik u ishrani ovnova, na neke parametre semena. Eksperiment je obavljen sa 15 klinički zdravih ovnova severnoistočne bugarske merino rase. Životinje su podeljene u tri grupe (5 po grupi). Ovnovi prve eksperimentalne grupe (G1) dobijali su obrok sa dodatkom 4,0 mg natrijum selenita (Na2SeO3) po grlu dnevno, dok su životinje druge eksperimentalne grupe (G2) hranjene obrokom sa 1,83 g L-selenometionina (Sel- Plek, Alltech, SAD) po grlu dnevno. Na kraju, svaka životinja iz G1 i G2 dobijala je 1,83g selena dnevno. Kontrolna grupa (GC) dobila je obrok bez dodatka selena. Glavni sastav obroka u svakoj grupi bio je isti. Ejakulati su dobijeni preko veštačke vagine. Ocenjivani su sledeći parametri: volumen i pH ejakulata i pokretljivosti, koncentracija i preživljavanje in vitro spermatozoida na 39°C tokom 360 min. Utvrđeno je da dodatak obroku neorganskog i organskog selena doveo do povećanja zapremine ejakulata, pokretljivosti i preživljavanja spermatozoida. Na sveže dobijeno seme nije uticao tretman sa selenom. |
