THE ROLE OF NITROXOLINE IN THE CONTROL OF UROPATHOGENIC ENTEROCOCCI INFECTIONS
| Autor: | Repac Antić, Davorka |
|---|---|
| Přispěvatelé: | Gobin, Ivana |
| Jazyk: | chorvatština |
| Rok vydání: | 2022 |
| Předmět: |
Urinary Tract Infections--drug therapy
nitroksolin Medicina Enterococcus faecalis--drug effects Drug Resistance gentamicin Medical sciences mesh:D013293 biofilm URINARNE INFEKCIJE antimikrobna rezistencija BIOMEDICINE AND HEALTHCARE. Clinical Medical Sciences. Medical Microbiology Anti-Bacterial Agents--therapeutic use enterococci nitroxoline mesh:D014552 udc:61(043.3) ENTEROCOCCUS FAECALIS antimicrobial resistance OTPORNOST NA LIJEKOVE hidrokinon mesh:D000900 hydroquinone enterokoki mesh:D004351 BIOMEDICINA I ZDRAVSTVO. Kliničke medicinske znanosti. Medicinska mikrobiologija ANTIBAKTERIJSKI LIJEKOVI |
| Popis: | Ciljevi istraživanja: Utvrditi mehanizam antimikrobnog i antiadhezijskog učinka nitroksolina (5-nitro-8-hidroksikinolin) na uropatogene enterokoke. Ispitati interakciju između nitroksolina s hidrokinonom ili gentamicinom i njihov utjecaj na spriječavanje stvaranja biofilma kao i na liječenje urinarnih infekcija uzrokovanih enterokokom. Metode: U radu su korištena 29 klinička izolata uropatogenih sojeva Enterococcus faecalis, te standardni ATCC 29212 soj. Klinički izolati su karakterizirani različitim biokemijskim metodama, 16S rRNA sekvencioniranjem, proteinskim profilom (MALDI TOF) te sposobnošću stvaranja biofilma. Antimikrobna učinkovitost se ispitivala određivanjem minimalne inhibicijske koncentracije (MIK), minimalne baktericidne koncentracije (MBK) i minimalne antiadhezijske koncentracije (MAK). Proteinski profil enterokoka prije i nakon tretmana se ispitao pomoću MALDI-TOF MS analize, dok su se morfološke promjene bakterijskih stanica nakon tretmana pratile transmisijskom elektronskom mikroskopijom (TEM), a promjene u strukturi biofilma pomoću pretražne elektronske mikroskopije. Rezultati: Testirani uropatogeni enterokoki pokazuju sličan biokemijski i proteinski profil, a razlikuju se prema svojstvu stvaranja biofilma te osjetljivosti na odabrana antimikrobna sredstva. Nitroksolin pokazuje bakteriostatsko i antiadhezijsko djelovanje na uropatogene enterokoke koje se u kombinaciji s hidrokinonom ili gentamicinom dodatno pojačava. Većina kombinacija hidrokinona i nitroksolina kao i gentamicina i nitroksolina pokazuje aditivni učinak na sve sojeve enterokoka. Zaključak: Navedene kombinacije mogle bi postati potencijalno terapijsko rješenje za liječenje urinarnih infekcija uzrokovanih enterokokima, no neophodno je detaljnije istražiti mehanizme djelovanja. Research objectives: To determine the mechanism of the antimicrobial and anti adhesion effect of nitroxoline (5-nitro-8-hydroxyquinoline) on uropathogenic enterococci. To examine the interaction between nitroxoline with hydroquinone or gentamicin and their effect on the prevention of biofilm formation as well as on the treatment of urinary infections caused by Enterococcus. Methods: In our research, 29 clinical isolates of uropathogenic E. faecalis strains and the standard ATCC 29212 strain were used. Clinical isolates were characterized by various biochemical methods, 16S rRNA sequencing, protein profile (MALDI-TOF) and the ability to form biofilm. Antimicrobial efficiency was tested by determining the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum antiadhesion concentration (MAC). The protein profile of enterococci before and after treatment was examined using MALDI-TOF MS analysis, while the morphological changes of bacterial cells after treatment were monitored by transmission electron microscopy (TEM), and changes in the biofilm structure using scanning electron microscopy (SEM). Results: The tested uropathogenic enterococci show a similar biochemical and protein profile and differ by ability to form biofilm as well as sensitivity to selected antimicrobial agents. Nitroxoline exhibits bacteriostatic and anti-adhesion effects on uropathogenic enterococci, which is further enhanced when combined with hydroquinone or gentamicin. Most combinations of hydroquinone and nitroxoline aswell as gentamicin and nitroxoline show an additive effect on all strains of enterococci. Conclusion: The mentioned combinations could become a potential therapeutic solution for the treatment of urinary infections caused by enterococci, but it is necessary to further investigate the mechanisms of action |
| Databáze: | OpenAIRE |
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