Determination of volatile compounds from freeze-dried and spray-dried samples of thyme, oregano, sage and rosemary : bachelor thesis
| Autor: | Jurić, Viktorija |
|---|---|
| Přispěvatelé: | Bilušić, Tea |
| Jazyk: | chorvatština |
| Rok vydání: | 2018 |
| Předmět: |
in vitro probava
metoda po Folin-Ciocalteu majčina dušica phenols PRIRODNE ZNANOSTI. Kemija Rosmarinus officinalis L Labiatae NATURAL SCIENCES. Chemistry hlapljivi spojevi Thymus vulgaris usnače in-vitro digestion volatile compounds spray-drying Salvia officinalis antioksidansi Lamiaceae mravinac Folin-Ciocalteu method ružmarin sušenje sprejem divlji mažuran HS-SPME/GC-MS fenoli liofilizacija antioxidants sage thyme freeze-drying kadulja oregano rosemary Origanum vulgare origano timijan |
| Popis: | Kadulja, origano, ružmarin i timijan se zbog svog bogatog sastava biološki aktivnih tvari koriste u prehrani. Mnoga istraživanja koja su provedena potvrđuju njihova antioksidativna, protuupalna i antivirusna djelovanja na čovjekov organizam. Prikupljeni biljni materijal podvrgnut je postupku liofilizacije i sušenja sprejem. Potom je koristeći HS-SPME/GC-MS tehniku određen udio hlapljivih komponenti. Također, spektrofotometrijskom metodom Folin- Ciocalteu određen je udio ukupnih fenola prije i poslije simuliranog dvofaznog modela probave s ljudskim probavnim sokovima (želudac i tanko crijevo). Dobiveni rezultati pokazuju da se kemijski sastav hlapljivih komponenti navedenih biljaka razlikuje s obzirom na vrstu sušenja – liofilizaciju i sušenje sprejem. Neke su se komponente obradom sušenja sprejem bitno povećale, dok su se neke druge komponente smanjile ili u potpunosti iščezle. Na temelju toga moguće je zaključiti da način sušenja biljnog materijala suvremenim tehnikama, poput postupka liofilizacije i sušenja sprejem, uvelike mijenja kemijski sastav hlapljivih komponenti. Nadalje, udio ukupnih fenola u biljnim uzorcima značajno je veći kod uzoraka osušenih sprejem u odnosu na liofilzirani biljni materijal. Što se tiče gastrointestinalne stabilnosti, iz rezultata je vidljivo da je udio fenola veći u duodenalnoj fazi probave u odnosu na želučanu fazu probave. Sage, oregano, rosemary, and thyme represent a rich source of biological active compounds (so called phytochemicals) and because of that they are very common in health diet regime. Many studies proved their numerous biological potential such as antioxidative, antibacterial, antiviral and anti-inflammatory. Collected plant samples have been processed using two drying techniques – freeze-drying and spray-drying. Using HS-SPME/GC- MS technique, the composition of volatile compounds has been determined. In addition, the amount of total phenolics was detected using spectrophotometric method Folin-Ciocalteu. The amount of total phenols was determined before and after simulated two-phase digestion process using human digestive enzymes (gastric and duodenal). Obtained results showed that chemical composition of volatile compounds was different in relation to plant material and in relation to different drying technique – freeze-drying and spray-drying. The concentration of some compounds was higher after spray-drying in relation to freeze-drying, while the concentration of other compounds was lower. Generally, it is possible to conclude that the composition of volatile compounds depends on the drying technique. In addition, the amount of total phenolics was significantly higher in spray-dried plant samples in relation to freeze-dried samples. Also, their gastrointestinal stability was higher in duodenal phase in relation to gastric phase. |
| Databáze: | OpenAIRE |
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