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Elektrokemijske tehnike karakterizira visoka osjetljivost, selektivnost, niska granica detekcije i granica kvantifikacije te su zbog toga prikladne za analizu različitih vrsta uzoraka. Cilj ovog diplomskog rada je primijeniti dvije elektrokemijske tehnike, cikličku i pravokutnovalnu voltametriju za karakterizaciju elektrokemijske oksidacije rutina te cikličku voltametriju kao tehnike za elektrokemijsko istraživanje stabilnosti modela rutin—β-glukan. Za elektrokemijsko istraživanje rutina i modela rutin—β-glukan koristila se standardna troelektrodna ćelija i inertni puferi različite pH vrijednosti, dok se stabilnost modela pratila kroz određeni vremenski period. Rutin je izabran kao jedan od predstavnika polifenolnih spojeva, flavonoida široko rasprostranjenih u biljkama te β-glukan kao jedan od predstavnika prehrambenih vlakana. Rezultati su pokazali da je elektrokemisjka oksidacija rutina reverzibilnate da na ponašanje ispitanog modela utječu pH otopine i vrijeme istraživanja. Electrochemical techniques are characterized by high sensitivity, selectivity, low limit of detection and limit of quantification and are therefore suitable for the analysis of different types of samples. The aim of this work was to apply two electrochemical techniques, cyclic and square-wave voltammetry to characterize the electrochemical oxidation of rutin and cyclic voltammetry as techniques for electrochemical investigation of the stability of the rutin—β-glucan model. For electrochemical investigation of rutin and rutin—β-glucan model, a standard three-electrode cell and inert buffers of different pH values were used, while the stability of the model was monitored over a time period. Rutin was chosen as one of the representatives of polyphenolic compounds, flavonoids widely distributed in plants and β-glucan as one of the representatives of dietary fibre. The results showed that the electrochemical oxidation of rutin was reversible so that the behaviour of the investigated model was influenced by the pH of the solution and the time of the study. |
