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Zadatak rada je ispitati utjecaj koncentriranja tvari boje postupkom reverzne osmoze pri različitim procesnim parametrima tlaka i temperature na uzorcima konvencionalnog i ekološkog vina Cabernet Sauvignon. Proces koncentriranja provodio se pri tlakovima od 25, 35, 45 i 55 bara, s primjenom hlađenja i bez primjene hlađenja, na laboratorijskom uređaju LabUnit M20 s pločama i okvirima, primjenom kompozitnih membrana tipa Alfa Laval – NFM20. Uzorci permeata i retentata uzimat će se u određenim vremenskim intervalima te će se odrediti: polifenoli, antocijani i antioksidacijska aktivnost upotrebom spektrofotometra te fenolne kiseline tekućinskom kromatografijom visoke djelotvnornosti na uređaju HPLC 1260 Infinity II (Agilent Technologies, CA, USA). Osnovni kemijski sastav odredit će se prema Pravilniku o fizikalno – kemijskim metodama analize mošta, vina, drugih proizvoda od grožđa i vina te voćnih vina, NN 106/2004. Project task is testing effect of concentrating substance of color by procedure of reverse osmosis with different pressure and temperature processing parameters on samples of conventional and ecological vine Cabernet Sauvignon. Concentrating process was conducted at pressures of 25, 35, 45 and 55 bars, with and withouth cooling application, on laboratory device LabUnit M20 with plates and frames, using composit membranes type Alfa Laval – NFM20. Permeate and retentate samples will be taken in certain time intervals and it will determine: polyphenols, anthocyanins and antioxidant activity using spectometer and also phenolic acid by high-performance liquid chromatography on HPLC 1260 Infinity II device ( Agilent Technologies, CA, USA). Basic chemical composion will be determined according to Regulations of physicochemical analyisis metod of must, vines, other grape products and vines and fruit vine, NN 106/2004. |
