Detection of Salmonella antibodies in the saliva of pigs from Salmonella Typhimurium-vaccinated and unvaccinated herds
| Autor: | DE LUCIA, ALESSIA, A. Rabie, R. P. Smith, R. Davies, C. Bianco, F. Ostanello, F. Martelli |
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| Přispěvatelé: | K. Nechvátalová, H. Nathues, De Lucia, A., Rabie, A., Smith, R. P., Davies, R., Bianco, C., Ostanello, F., Martelli, F. |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Popis: | This study aimed to investigate the oral fluid saliva (OF) collected passively via chewed sampling ropes as a potential sample type for assessing anti-Salmonella antibodies in 2 Salmonella-vaccinated (V) and 2 non- vaccinated (NV) pig farms, comparing the results with direct bacteriological diagnostic methods. Gilts and sows in the V farms were vaccinated prior to farrowing with a live attenuated vaccine (Salmoporc STM - IDT Biologika). For each age class (sows - S, weaners - W and grower pigs – G) pooled of faecal and OF samples were collected. Salmonella was isolated according to a modification of ISO6579 Annex D. In parallel, IgG antibody titres were assessed in OF samples using an ELISA assay (IDEXX Swine Salmonella Ab Test - IDEXX Laboratories). Salmonella was detected in 90.6% of faecal samples in NV farms, and in 36.1% of V farms (p |
| Databáze: | OpenAIRE |
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