Beet soil-borne mosaic virus RNA-4 encodes a 32 kDa protein essential for virus transmission through Polymyxa betae

Autor: D’Alonzo M., A. Delbianco, C. Lanzoni, C. Rubies Autonell, D. Gilmer, RATTI, CLAUDIO
Přispěvatelé: D’Alonzo M., A Delbianco, C Lanzoni, C. Rubies Autonell, D Gilmer, C. Ratti
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Popis: Beet soil-borne mosaic virus (BSBMV), like Beet necrotic yellow vein virus (BNYVV), is a member of the Benyvirus genus and both are transmitted by Polymyxa betae. Both viruses possess a similar genomic organization: RNA-1 and -2 are essential for infection and replication while RNA-3 and -4 play important roles in disease development and vector-mediated infection in sugar beet roots. We characterized a new species of BSBMV RNA-4 that encodes a 32 kDa protein and a chimeric form of BSBMV RNA-3 and -4. We demonstrated that BSBMV RNA-4 can be amplified by BNYVV RNA-1 and -2 in planta, is involved in symptoms expression on Chenopodium quinoa plants and can also complement BNYVV RNA-4 for virus transmission through its vector P. betae in Beta vulgaris plants. Using replicon-mediated expression, we demonstrate for the first time that a correct expression of RNAs-4 encoded proteins is essential for benyvirus transmission.
Databáze: OpenAIRE