| Autor: |
DARESTA, MARCO, FELICE, VIVIANA, PANARESE, SERENA, BIANCO, CARLO, BACCI, MARIA LAURA, SARLI, GIUSEPPE, OSTANELLO, FABIO, DOTTORI M., BONILAURI P., LELLI D. |
| Přispěvatelé: |
DARESTA M., FELICE V., PANARESE S., BIANCO C., BACCI M.L., DOTTORI M., BONILAURI P., LELLI D., SARLI G., OSTANELLO F. |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
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| Popis: |
From previous investigations a low antibody titre and a prolonged viremia in non-vaccinated compared to vaccinated gilts revealed to be the higher risk factor associated to fetus infection in conventional animals inseminated with PCV2 spiked semen. A trial was performed to compare antibody titres, viremia, fetuses and fetal membranes/fluids samples PCV2 positivity in four groups of conventional gilts: 6 vaccinated with a commercial inactivated PCV2 vaccine (VAI), 6 vaccinated with a commercial vaccine based on a ORF2 capsid protein expressed in a baculovirus system and licensed for use in piglets (VBI), 6 non vaccinated (NVI) and 3 non-vaccinated and non-infected controls (CTR). Both types of vaccines were administered in gilts at 120 and 150 days of life. All animals received Regumate® for 18 days followed by an estrus syncronization and superovulation protocol. VAI, VBI and NVI groups were inseminated with a double (24 hrs apart) dose of PCV2 negative semen spiked with a PCV2b strain isolated in a PMWS outbreak in Italy. CTR gilts were fecundated with a double dose of PCV2 free semen. PCV2 in tissues was assessed and quantified by real time PCR. In the VAI group 4 out of 6 gilts were pregnant, in VBI 3 out of 6, in NVI 3 out of 6 and in CTR 2 out of 3, from which were collected 25, 19, 33 and 20 fetuses, respectively and corresponding placenta membranes and amniotic fluid. No differences in antibody titres nor viremia were revealed among groups during the trial. CTR showed the significantly (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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