Flexible and Hollow Micro Ring Electrode Arrays for Multi-Directional Monitoring of 3D Neuronal Networks
| Autor: | Suresh Vajrala, Venkata, Eddarir, Asma, Pautot, Sophie, Bergaud, Christian, Maziz, Ali |
|---|---|
| Přispěvatelé: | Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Équipe Microsystèmes électromécaniques (LAAS-MEMS), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | 2022 MRS Spring Meeting & exhibit 2022 MRS Spring Meeting & exhibit, May 2022, Honololu, Hawaii, United States |
| Popis: | Three-dimensional (3D) cell culture based in vitro models shown great promise and able to replicate partially the development and complexity of in vivo nerve tissue. The functional characterization of 3D cell cultures is challenging. Because, most of the in vitro neural interfacing technologies are developed for 2D cell cultures, where it limits the access to the networks within the cultures. Hence, high-performance electrophysiological platforms that allow seamless integration with soft and stable tissue system, and multi-directional bio interfacing capabilities for long term are required.In this context, we report for the first time the fabrication of ‘Stargate’ look alike flexible and hollow micro-ring electrode array (SG MEA) with hole at the centre (40-μm to 150-μm diameter). The SG MEA was microfabricated on a 20-μm thick layer of flexible and biocompatible parylene C configured with 4 to 6 electrode sites. All gold electrode rings were coated with the conducting polymer poly(3,4-ethylenedioxythio-phene):poly(styrene-sulfonate) (PEDOT:PSS) to lower the impedance (5-8 kΩ) and obtain a better signal-to-noise ratio during extracellular recordings. The hollow ring shape of SG electrode architectures, with geometrical surface area of 289 μm2 to 1299 μm2 were designed specifically in such a way that it offers more freedom to the internal dynamics of cell cultures and multidirectional sensing of neuron activities within the 3D neuronal network, from both front and back-end of the SG MEA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|