Innate Memory Reprogramming by Gold Nanoparticles Depends on the Microbial Agents That Induce Memory
| Autor: | Swartzwelter, Benjamin J., Michelini, Sara, Frauenlob, Tobias, Barbero, Francesco, Verde, Alessandro, De Luca, Anna Chiara, Franco Puntes, Victor |
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| Přispěvatelé: | Institut Català de la Salut, [Swartzwelter BJ] Institute of Biochemistry and Cell Biology (IBBC), National Research Council (CNR), Napoli, Italy. Department Biosciences, Paris Lodron University of Salzburg (PLUS), Salzburg, Austria. [Michelini S, Frauenlob T] Department Biosciences, Paris Lodron University of Salzburg (PLUS), Salzburg, Austria. [Barbero F] Institut Català de Nanociència i Nanotecnologia (ICN2), Consejo Superior de Investigaciones Científicas (CSIC) and The Barcelona Institute of Science and Technology (BIST), Barcelona, Spain. [Verde A, De Luca AC] Institute of Biochemistry and Cell Biology (IBBC), National Research Council (CNR), Napoli, Italy. [Puntes V] Institut Català de Nanociència i Nanotecnologia (ICN2), Consejo Superior de Investigaciones Científicas (CSIC) and The Barcelona Institute of Science and Technology (BIST), Barcelona, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Institució Catalana de Recerca I Estudis Avançats (ICREA), Barcelona, Spain, Vall d'Hebron Barcelona Hospital Campus |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Otros calificadores::Otros calificadores::/administración & dosificación [Otros calificadores]
Memòria - Efecte dels medicaments Nanopartícules Hemic and Immune Systems::Blood::Blood Cells::Leukocytes::Leukocytes Mononuclear::Hemic and Immune Systems::Hemic and Immune Systems::Hemic and Immune Systems::Hemic and Immune Systems::Monocytes [ANATOMY] Monòcits - Immunologia Technology Industry and Agriculture::Manufactured Materials::Nanostructures::Nanoparticles::Metal Nanoparticles [TECHNOLOGY INDUSTRY AND AGRICULTURE] Other subheadings::Other subheadings::/administration & dosage [Other subheadings] sistemas sanguíneo e inmunológico::sangre::células sanguíneas::leucocitos::leucocitos mononucleares::sistemas sanguíneo e inmunológico::sistemas sanguíneo e inmunológico::sistemas sanguíneo e inmunológico::sistemas sanguíneo e inmunológico::monocitos [ANATOMÍA] Immune System Phenomena::Immunity::Adaptive Immunity::Immunologic Memory [PHENOMENA AND PROCESSES] fenómenos del sistema inmunitario::inmunidad::inmunidad adaptativa::memoria inmunológica [FENÓMENOS Y PROCESOS] tecnología industria y agricultura::productos manufacturados::nanoestructuras::nanopartículas::nanopartículas metálicas [TECNOLOGÍA INDUSTRIA Y AGRICULTURA] |
| Zdroj: | Scientia |
| Popis: | Inmunidad innata; Agentes microbianos; Nanopartículas Innate immunity; Microbial agents; Nanoparticles Immunitat innata; Agents microbians; Nanopartícules Innate immune memory, the ability of innate cells to react in a more protective way to secondary challenges, is induced by exposure to infectious and other exogeous and endogenous agents. Engineered nanoparticles are particulate exogenous agents that, as such, could trigger an inflammatory reaction in monocytes and macrophages and could therefore be also able to induce innate memory. Here, we have evaluated the capacity of engineered gold nanoparticles (AuNPs) to induce a memory response or to modulate the memory responses induced by microbial agents. Microbial agents used were in soluble vs. particulate form (MDP and the gram-positive bacteria Staphylococcus aureus; β-glucan and the β-glucan-producing fungi C. albicans), and as whole microrganisms that were either killed (S. aureus, C. albicans) or viable (the gram-negative bacteria Helicobacter pylori). The memory response was assessed in vitro, by exposing human primary monocytes from 2-7 individual donors to microbial agents with or without AuNPs (primary response), then resting them for 6 days to allow return to baseline, and eventually challenging them with LPS (secondary memory response). Primary and memory responses were tested as production of the innate/inflammatory cytokine TNFα and other inflammatory and anti-inflammatory factors. While inactive on the response induced by soluble microbial stimuli (muramyl dipeptide -MDP-, β-glucan), AuNPs partially reduced the primary response induced by whole microorganisms. AuNPs were also unable to directly induce a memory response but could modulate stimulus-induced memory in a circumscribed fashion, limited to some agents and some cytokines. Thus, the MDP-induced tolerance in terms of TNFα production was further exacerbated by co-priming with AuNPs, resulting in a less inflammatory memory response. Conversely, the H. pylori-induced tolerance was downregulated by AuNPs only relative to the anti-inflammatory cytokine IL-10, which would lead to an overall more inflammatory memory response. These effects of AuNPs may depend on a differential interaction/association between the reactive particle surfaces and the microbial components and agents, which may lead to a change in the exposure profiles. As a general observation, however, the donor-to-donor variability in memory response profiles and reactivity to AuNPs was substantial, suggesting that innate memory depends on the individual history of exposures. This work was supported by the EU Commission H2020 projects PANDORA (GA 671881) and ENDONANO (GA 812661), the Italian MIUR InterOmics Flagship projects MEMORAT and MAME, the Italian MIUR/PRIN-20173ZECCM, the Priority program ACBN (Allergy Cancer BioNano Research Centre) of the University of Salzburg, the Cancer Cluster Salzburg, the Research Grant from the University of Salzburg, and the Austrian Science Fund (FWF) Grant Nr. P 29941. |
| Databáze: | OpenAIRE |
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