| Autor: |
De Vos, Michel, Ortega, Carolina, Smitz, Johan, Van Vaerenbergh, Inge, Bourgain, Claire, Devroey, Paul |
| Přispěvatelé: |
Department of Embryology and Genetics, Pathological Anatomy, Follicle Biology, Reproduction and Genetics |
| Jazyk: |
angličtina |
| Rok vydání: |
2011 |
| Předmět: |
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| Popis: |
Introduction: Despite recent technical advances to the laboratory protocol of in vitro maturation of oocytes (IVM), implantation rates after IVM are still lower than in conventional ART. Typically, the abrogated follicular phase and the absence of functional corpora lutea compromise the follicular and luteal sex steroid production and preclude the development of a receptive endometrium. Therefore, most clinical IVM protocols rely on the use of estrogen and progesterone supplementation for endometrial preparation. However, studies focusing on the implantation potential of the endometrium in IVM-cycles are scarce. Material and Methods: Between February 2009 and December 2010, endometrium quality in the luteal phase of IVM cycles was prospectively assessed by a single observer using vaginal ultrasound scanning and histological analysis of endometrium biopsies performed on day 5 or 6 after immature oocyte retrieval (OR). This schedule would coincide with the transfer of a cleavage-stage embryo after IVM and ICSI. 67 healthy egg donors (54 with regular cycles and 13 with polycystic ovarian syndrome (PCOS)) and 18 patients with PCOS, who underwent elective freezing of all embryos, underwent a total of 100 IVM cycles. Endometrial priming consisted of 450 IU uFSH or HP-HMG, followed by 6 mg daily transdermal estradiol gel (10 mg if endometrium thickness was |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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