| Autor: |
Potters, D., Van Moer, Guy, Aerts, Martine, De Pauw, Aline, Jochmans, Kristin, De Waele, Marc |
| Přispěvatelé: |
Immunology and Microbiology |
| Jazyk: |
angličtina |
| Rok vydání: |
1997 |
| Předmět: |
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| Popis: |
The influence of sample storage on the numbers of blood cells and on the differential count obtained with the Abbott Cell-Dyn CD4000 cell counter was examined. Blood samples of healthy volunteers were collected on K2EDTA. Cell counting was performed immediately after collection and after different periods of storage (up to 72 hours) at room temperature (RT) and at 4°C. Samples stored at 4°C were brought to RT before examination. The white blood cell (WEC) counts and the differential counts were compared to those obtained with the Abbott CelI-Dyn 3500 cell counter. With the CD 4000 the number of red blood cells, the hemoglobin concentration and the number of reticulocytes remained constant during the 72 hours of storage at both temperatures. The MCV increased after 8 hours of storage at RT but remained unchanged at 4°C. The platelet counts remained unchanged when the impedance method (PIC) was used. With the optical method the counts decreased after 4 hours of storage at RT and after 48 hours at 4°C. The WEC count decreased slightly in a minority of the samples after 56 hours at RT. This was accompanied by an increase of cells considered as eryrhroblasts. The viable fraction of the WBC decreased after 8 hours of storage at RT and reached 50% of the cells after 72 hours. After 8 hours at RT the segmented neutrophils decreased, while band forms and immature granulocytes appeared. This phenomenon reached a peak value and then partially disappeared again. Lymphocyte numbers decreased after 48 hours of storage at RT while cells classified as atypical lymphocytes appeared. All these phenomena were retarded by storage of the samples at 4°C instead of at RT. The high stability of the WBC count upon storage seen with the CD 4000 was comparable to that found with the impedance method (WIC) of the CD 3500 cell counter, while the optical method (WOC) showed a decrease of the white blood cells after 8 hours of storage at RT. In addition, a progressive shift of cells from the neutrophilic to the Iymphocytic compartment was seenwith the latter cell counter. In conclusion, little influence was found of sample storage up to 72 hours on the number of red blood cells, the hemoglobin concentration, the platelet count (PIC value), and the WBC count determined with the CD 4000 Abbott cell counter. Changes in MCV and differential count could be retarded by storage of the samples at 4°C instead of at RT. The differential count was more stable than that found with the Abbott Cell-Dyn 3500. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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